Yong Dai
Jinan University, China
Title: Diversity and clonality of B-cell and T-cell receptors in IgA nephropathy
Biography
Biography: Yong Dai
Abstract
Immunoglobulin A nephropathy (IgAN) is the most common cause of glomerular hematuria and one of the leading causes of end-stage renal disease (ESRD). Immune dysregulation contributes to this fatal disorder. Therefore, the receptor of the immune cells (mainly B/T Cells) may be valuable for the development of specific immunotherapeutic interventions. We isolated B cells and T cells from peripheral blood of IgAN patients and healthy controls and analyzed the composition of the BCR/TCR CDR3 by multiplex PCR, high-throughput sequencing and bioinformatics. The results revealed that the BCR/TCR CDR3 clones were expressed at very low frequencies, and the composition of clone types in IgAN patients was skewed; vast majority of clones were unique. Only 12 BCR and 228 TCR CDR3 clones were public clones, of which 16 public clones were expressed at a significantly higher frequency in IgAN patients (P<0.001). There were also specific conserved amino acid residues between these unique clones or groups, and the residues GMDV, EQY and EQF were recurring only in the IgAN group. Besides, some VDJ gene recombinations showed considerable variation between groups, including 4 high-frequency VDJ gene recombinations in the IgAN patients (P<0.001). Immune repertoires provide tremendous novel information, and conserved BCR/TCR CDR3 clones and VDJ gene recombinations with great variation may be potential therapeutic targets for IgAN patients.